Rapid ultrasonic isothermal amplification of DNA with multiplexed melting analysis – applications in the clinical diagnosis of sexually transmitted diseases.
نویسندگان
چکیده
We describe a nucleic acid testing (NAT) platform for infectious disease diagnostics at the point-of-care, using surface acoustic waves (SAW) to perform a multiplexed loop-mediated isothermal amplification (LAMP) test for sexually transmitted diseases. The ultrasonic actuation not only enables faster NAT reactions but also provides a route towards integrating low-cost, low-power molecular diagnostics into disposable sensors.
منابع مشابه
A capillary-based multiplexed isothermal nucleic acid-based test for sexually transmitted diseases in patients† †Electronic supplementary information (ESI) available: Additional methods and experimental sections. See DOI: 10.1039/c6cc05679b Click here for additional data file.
We demonstrate a multiplexed loop mediated isothermal amplification (LAMP) assay for infectious disease diagnostics, where the analytical process flow of target pathogens genomic DNA is performed manually by moving magnetic beads through a series of plugs in a capillary. Heat is provided by a water bath and the results are read by the naked eye, enabling applications in low resource settings.
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ورودعنوان ژورنال:
- Chemical communications
دوره 51 13 شماره
صفحات -
تاریخ انتشار 2015